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KMID : 0368419970400010021
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Journal of Plant Biology
1997 Volume.40 No. 1 p.21 ~ p.26
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Effect of Ammonium lon on Morphogenesis from Cultured Cotyledon Explants of Panax ginseng
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Choi Yong-Eui
Soh Woong-Young
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Abstract
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Cotyledon explants of Panax ginseng were cultured on modified Murashige and Skoog medium with various concentrations of NH_4Cl and KNO_3. Morphogenesis such as somatic embryo, embryogenic callus, or adventitious root formation from cotyledon explants differently occurred according to the concentrations of NH_4^+ and NO_3^-. Somatic embryos were actively formed in a moderate concentration of NH_4^+ (20 mM) in combination of NO_3^-, but in a high concentration of NH_4^+ (60 mM), only embryogenic calli were formed. In little or no NH_4^+, adventitious roots were formed at a high rate. The influence of NO_3^- on those morphogenesis was slight but combination of NO_3^- with NH_4^+ was indispensable since the cotyledon explants were necrotized on medium containing only NH_4^+ as a nitrogen source. Histological observation revealed that somatic embryo and embryogenic callus formation occurred from the same origin (cotyledon epidermis), whereas, adventitious roots were originated from the cells near vascular strands.
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